Are you a chemistry student? Visit A-Level Chemistry to download comprehensive revision materials - for UK or international students!

Thin layer chromatography

Introduction to thin layer chromatography (TLC) Chromatography is an analytical technique used to separate mixture of chemical substances into its individual compounds. Different types of chromatography are used in lab. e.g. column chromatography, thin-layer chromatography, gas chromatography etc. Among these, thin layer chromatography (TLC) is the most widely used method in chemical or biochemical laboratory. … Read more

Chromatography

Definition of chromatography Chromatography is an analytical technique used to separate mixture of chemical substances into its individual compounds. Different types of chromatography are used in lab. e.g. column chromatography, thin-layer chromatography, gas chromatography etc. Principles of chromatography Chromatography consists of two phases: one mobile phase and one contiguous stationery phase. The stationery phase is … Read more

High Performance Liquid Chromatography (HPLC)

HPLC Basics High performance liquid chromatography or commonly known as HPLC is an analytical technique used to separate, identify or quantify each component in a mixture. The mixture is separated using the basic principle of column chromatography and then identified and quantified by spectroscopy. A computer analyzes the data show the output in display. HPLC … Read more

Mass Spectrometry

Mass Spectrometry Definition Mass spectrometry is a powerful analytical technique used to know the molecular mass of unknown compound, quantify the known compound and elucidate the structure of a compound. Mass spectrometry convert the sample into gaseous ions with or without fragmentation and then characterize the sample with mass and charge ratios (m/z) and their relative … Read more