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Gas chromatography

Introduction to gas chromatography Gas chromatography is a chromatography technique that can separate and analyze volatile compounds in gas phase. Depending on stationary phase used in this analytical technique, there are two types of gas chromatography: Gas-liquid chromatography (GLC) Gas-solid chromatography (GSC). Among these, GLC is most widely used method. A gas chromatography looks like: … Read more

Paper chromatography

Introduction to paper chromatography Paper chromatography is a chromatography technique used to separate mixture of chemical substances into its individual compounds. Paper chromatography is used to teach TLC or other chromatography as it is very similar to TLC. Principles of paper chromatography All chromatography follow the same principle. Paper Chromatography consists of two phases: one … Read more

Column chromatography

Introduction to column chromatography Column chromatography is a chromatography technique used to separate mixture of chemical substances into its individual compounds. Column chromatography is a widely used method for the purification or separation of chemical compound mixture in lab. Principles of column chromatography Column Chromatography consists of two phases: one mobile phase and one contiguous … Read more

Thin layer chromatography

Introduction to thin layer chromatography (TLC) Chromatography is an analytical technique used to separate mixture of chemical substances into its individual compounds. Different types of chromatography are used in lab. e.g. column chromatography, thin-layer chromatography, gas chromatography etc. Among these, thin layer chromatography (TLC) is the most widely used method in chemical or biochemical laboratory. … Read more

Chromatography

Definition of chromatography Chromatography is an analytical technique used to separate mixture of chemical substances into its individual compounds. Different types of chromatography are used in lab. e.g. column chromatography, thin-layer chromatography, gas chromatography etc. Principles of chromatography Chromatography consists of two phases: one mobile phase and one contiguous stationery phase. The stationery phase is … Read more

High Performance Liquid Chromatography (HPLC)

HPLC Basics High performance liquid chromatography or commonly known as HPLC is an analytical technique used to separate, identify or quantify each component in a mixture. The mixture is separated using the basic principle of column chromatography and then identified and quantified by spectroscopy. A computer analyzes the data show the output in display. HPLC … Read more